Food product are now being enriched and fortified with vitamins in many forms. But does the amount present in the food at the food at the end of the shelf life match the label package?
Food manufacturers, regulatory agencies and commercial laboratories should therefore have analytical methods on hands that allow them to quickly and reliable determine the natural and added vitamin content of food products.
There are different methods for analysing water soluble vitamins: ELISA, Immunoaffinity Columns (IAC) and Microbiological Microtiter Plates. Notice that with the ELISA test system only added vitamins in simple matrices can be analysed. When using Immunoaffinity Columns in conjunction with HPLC, the sample is purified and the vitamin is retained by the antibody in the column. Using the Vitamin B12 and Biotin IAC, you can determine the total vitamin content. With the Folic Acid IAC, you only can determine added folic acid.