| No | SUBJECTS/TOPIC |
|---|---|
| Day 1 | |
| 1. | Introduction |
| 2. | History and Fundamentals of Microbiology |
| 3. | Rapid Tests Demonstrations - Presence/Absence with Bromcresol Purple Indicator - Paddle Tests - Biological Activity Reaction Tests (BARTs) |
| 4. | Sampling for Microbiology Analysis 3.1 Theory, Techniques, Importance & Application |
| 5. | Membrane Filtration Lecture and Demonstration |
| 6. | Lab 1 - m-Endo Broth media for potable water |
| 7. | Lab 2 - m-FC Broth for nonpotable water |
| 8. | Lab 3 - m-Coli Blue 24 Broth for nonpotable water |
| 9. | Most Probable Number (MPN) Lecture and Demonstration |
| 10. | Lab 4 - MPN on nonpotable water |
| 11. | Lab 6 - Presence/Absence, Collilert and Quanti Tray |
| 12. | Heterotrophic Bacteria Testing via Pour Plate Method |
| 13. | Lab 7 - HPC testing on non-potable water |
| Day 2 | |
| 1. | 5 Steps of "Microbiological" Analysis |
| 2. | Review of the tests parameters from Day 1 |
| 3. | Enumeration of Rapid Tests Demo and Discussion of Results |
| 4. | Discussion and Evaluation of Membrane Filtration Results |
| 5. | Lab 8 - Enumeration of Potable water samples |
| 6. | Lab 9 - Confirmation with Nutrient Agar with MUG |
| 7. | Lab 10 - Enumeration of Nonpotable water samples |
| 8. | Discussion of Microbial Induced Corrosion and BARTs |
| 9. | Discussion of MPN tests and record keeping |
| 10. | Lab 11 - Evaluation of MPN test and Confirmation |
| 11. | Discussion of HPC's |
| 12. | Lab 12 - Enumeration of Pour Plates |
| 13. | Confirmation counts of Total Coliforms with Nutrient Agar with MUG |
| 14. | Destruction of tests and Waste Management |