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Genetically Modified Organisms (GMO)

Real-Time PCR

REQUEST QUOTATION

These DNA sequences, mostly for insect, herbicide and/or insect resistance are enveloped in a frame of viral or bacterial DNA sequences which serves as promoters or terminators.

Different international and national legislations and labelling regulations requires multi-stage analysis, for which real-time PCR is the method of choice.

  • The presence of GMOs can be screened by identifying the genetic sequence elements as 35S, NOS and FMV. Further genetic elements may be expected in the future. 35S positive results should be confirmed for absence of natural contamination of the cauliflower mosaic virus using the CaMV system. Furthermore, the efficiency of the DNA preparation should be confirmed using plant DNA, when analysing a new matrix.
  • For GMO positive samples the identification of the GMO event is of main interest to classify the food product in approved or illegal GMO. In Europe the legislation EC 1829/2003 and 1830/2003 describes the relevant regulations. Non-approved GMO products are not allowed to enter or to be produced or processed in Europe.
  • A zero tolerance strategy is in force for Europe, while for feed samples a technical threshold of 0.1 % has been established (EC 618/2011). Food samples with a content of > 0.9 % approved GMO per matrix has to be labelled.

For approved GMOs in food samples quantification in the relevant range of approximately 0.9 % is of main interest. The GMO content in DNA copy numbers can be quantified relative to the plant matrix and the results will be given in percent.

Parameters available:

SureFood® PREP

DNA Preparation

Product

Plant

Description

DNA preparation from mainly plant based food and feed

No. of Tests / Amount

100 preparations

Code No.

S1002

 

Product

Plant X

Description

DNA preparation from highly processed plant based food and feed

No. of Tests / Amount

50 preparations

Code No.

S1006

 

SureFood® GMO

Qualitative Real-Time PCR

Product

Plant PLUS

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2049

 

Product

Plant

Description

Complementary kit to S2026 and S2126

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2056

 

Product

Plant 4plex Corn/Soya/ Canola/Cotton

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2156

 

Product

Plant 4plex Corn/Soya/ Canola + IAC

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2158

 

SureFood® GMO SCREEN 

Qualitative Real-Time PCR

Product

35S + NOS + FMV

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2026

 

Product

4plex 35S / NOS / FMV+ IAC

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2126

 

Product

35S

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2045

 

Product

NOS

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2046

 

Product

FMV

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2047

 

Product

BAR

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2048

 

Product

CaMV

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2027

 

Product

CTP2:CP4  EPSPS

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2059

 

Product

CryIAb

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2063

 

Product

P35S:BAR Rice

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

2 x 50 reactions

Code No.

S2022

** includes additional 100 reactions of Inhibition Control Mix (ICM); 100 reactions for each parameter

 

SureFood® GMO ID

Qualitative Real-Time PCR

Product

LibertyLink601 Rice

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

2 x 50 reactions

Code No.

S2023

 

Product

Bt63 Rice

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

2 x 50 reactions

Code No.

S2024

 

Product

Roundup Ready Soya

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2030

 

Product

MON810 Corn

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2033

 

Product

RR2Y Soya

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2034

 

Product

MIR162 Corn

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2035

 

Product

MON863 Corn

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2037

 

Product

MIR604 Corn

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2039

 

Product

A2704-12 Soya

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2057

 

Product

GT73 Canola

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2058

 

Product

T45 Canola

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2060

 

Product

T45 Canola

Description

Qualitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

100 reactions

Code No.

S2062

 

Product

CMS Ogura

Description

Qualitative DNA detetction of Ogura-sequence in cabbage

No. of Tests / Amount

100 reactions

Code No.

S2064

 

SureFood® GMO QUANT

Quantitative Real-Time PCR

Product

Roundup Ready Soya

Description

Quantitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

2 x 50 reactions*

Code No.

S2014

 

Product

Bt176 Corn

Description

Quantitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

2 x 50 reactions*

Code No.

S2015

 

Product

Bt11 Corn

Description

Quantitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

2 x 50 reactions*

Code No.

S2016

 

Product

T25 Corn

Description

Quantitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

2 x 50 reactions*

Code No.

S2017

 

Product

MON810 Corn

Description

Quantitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

2 x 50 reactions*

Code No.

S2019

 

Product

35S Corn

Description

Quantitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

2 x 50 reactions*

Code No.

S2020

 

Product

35S Soya

Description

Quantitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

2 x 50 reactions*

Code No.

S2028

 

Product

RR2Y Soya

Description

Quantitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

2 x 50 reactions*

Code No.

S2029

 

Product

NK603 Corn

Description

Quantitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

2 x 50 reactions*

Code No.

S2050

 

Product

MON863 Corn

Description

Quantitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

2 x 50 reactions*

Code No.

S2051

 

Product

GA21 Corn

Description

Quantitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

2 x 50 reactions*

Code No.

S2054

 

Product

GT73 Canola

Description

Quantitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

2 x 50 reactions*

Code No.

S2061

 

Product

MIR162 Corn

Description

Quantitative DNA detection

Detection limit: 0.01 % depending on matrix and DNA preparation

No. of Tests / Amount

2 x 50 reactions*

Code No.

S2135

* 1 x 50 reactions for the detection of the reference gene

 
 
 
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