IDEXX - USEPA approved method for detecting bacteria in water

Colilert® Coliforms and E.Coli (Quanti - Tray Sealer PLUS, Incubator)

An Easy 24 hour test for coliform and E.coli
Colilert has become the number one method in countries around the world because:

  • Colilert takes less than one minute hands-on time per sample
  • Colilert is approved internationally for compliance testing
  • Colilert has been shown to be 20-50% less expensive than traditional methods.

Colilert Benefits

  • US EPA-approved, 24-hour test for drinking and source waters.
  • International approvals include US EPA, Standard Methods for Examination of Water and Wastewater, AOAC, IBWA, and EBWA.
  • Millions of tests are run worldwide every year.
  • Ease of use simplifies training.
  • Unit-dosed packaging eliminates media preparation.
  • No repeat testing due to clogged filters or heterotrophic interference.
  • Quality Control (QC) procedure can be done in 15 minutes.
  • Under one minute hands-on time.
  • Detects coliforms and E. coli simultaneously in 24 hours or less.
  • No confirmations needed.
  • No glassware cleaning or colony counting.
  • Identifies E. coli specifically, which eliminates unneeded public notification due to Klebsiella pneumoniae.
  • Suppresses up to 2 million heterotrophs per 100 ml.
  • Eliminates the subjective interpretation found in traditional methods.
  • Detects a single viable coliform or E. coli per sample.
  • 20 to 50% less expensive than traditional methods. (According to AWWARF Research Application #4).
  • 95% lower equipment costs.
  • Minimizes evening and weekend work.
  • Up to 12-month shelf life at room temperature

1. Presence/ Absence
Add reagent to sample and incubate 24 hours at 35°C

Read results. Colerless = negative
Yellow = total coliforms
Yellow/fluorescent = E.Coli

2. Quantification
Add Reagent to sample and mix well

Pour into Quanti-Tray® (count from 1 to 200) or Quanti-Tray® (count from 1 to 2,419)

Seal in Quanti-Tray Sealer and place in oncubator for 18 hours at 35°C

  • Read Quanti-tray results.
    Yellow wells = total coliforms
    Yellow/Fluorescent wells = E.coli
    Refer to MPN table
  • Read Quanti-tray 2000 results.
    Yellow wells = total coliforms.
    Yellow/Fluorescent wells = E.coli
    Refer to MPN table